Sol Genomics Network

SGN Marker C2_At4g20410

SGN-M8307
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	301221 (SGN) 122421 (CGN)	-	-	-
Lycopersicon Combined	Single	SGN-U222292	-	-	-
Solanum tuberosum	Single	SGN-U245717	-	-	-
Arabidopsis	Single	At4g20410 on TAIR	-	-	-

Mapped locations

Map: Pepper-COSII
Map version 58 (location: 41479)
Chromosome 2
Position 47.30 cM
Confidence I(LOD2)
Protocol CAPS

Pepper-COSII

PCR data Exp. ID 43455

Forward primer (5'-3')

GCTTATCTTAGTGCAATCATTGT

Accessions and product sizes

Approximate temperature

55°C

Reverse primer (5'-3')

CCGTCAGCATATGCAGAAAGAAGT

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

Unknown

Enzymes

Map: Tomato-EXPEN 2000
Map version 52 (location: 38660)
Chromosome 2
Position 36.90 cM
Confidence I
Protocol CAPS

Tomato-EXPEN 2000

PCR data Exp. ID 39838

Forward primer (5'-3')

TGTGGGAGGCCCCAACCTGC

Accessions and product sizes

Solanum pennellii TA56

600

Solanum lycopersicum TA209

750

Approximate temperature

55°C

Reverse primer (5'-3')

TCAGGTGCTCCATCTTCTAAAGC

Digested band sizes (using amplicon difference)

Solanum lycopersicum TA209

750

Solanum pennellii TA56

600

Mg⁺² concentration

1.5mM

Enzymes

Map: Arabidopsis COSII
Map version 44 (location: 26747)
Chromosome 4
Position 11.02 MB
Confidence uncalculated
Protocol CAPS

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 11434
Forward primer (5'-3') TGTGGGAGGCCCCAACCTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAGGTGCTCCATCTTCTAAAGC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1350	Approximate temperature 55°C

PCR data Exp. ID 11435
Forward primer (5'-3') TGTGGGAGGCCCCAACCTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAGGTGCTCCATCTTCTAAAGC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1350	Approximate temperature 55°C

PCR data Exp. ID 11436
Forward primer (5'-3') TGTGGGAGGCCCCAACCTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAGGTGCTCCATCTTCTAAAGC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 1350	Approximate temperature 55°C

PCR data Exp. ID 13434
Forward primer (5'-3') TGTGGGAGGCCCCAACCTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAGGTGCTCCATCTTCTAAAGC	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 1300	Approximate temperature 55°C

PCR data Exp. ID 13435
Forward primer (5'-3') TGTGGGAGGCCCCAACCTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAGGTGCTCCATCTTCTAAAGC	Enzymes
Accessions and product sizes Capsicum annuum TA3428: 600+680	Approximate temperature 55°C

PCR data Exp. ID 13436
Forward primer (5'-3') TGTGGGAGGCCCCAACCTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAGGTGCTCCATCTTCTAAAGC	Enzymes
Accessions and product sizes Petunia axillaris TA3309: 1500	Approximate temperature 55°C

PCR data Exp. ID 13437
Forward primer (5'-3') TGTGGGAGGCCCCAACCTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAGGTGCTCCATCTTCTAAAGC	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: 1500	Approximate temperature 55°C

PCR data Exp. ID 13438
Forward primer (5'-3') TGTGGGAGGCCCCAACCTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAGGTGCTCCATCTTCTAAAGC	Enzymes
Accessions and product sizes Petunia integrifolia TA3312: 1500	Approximate temperature 55°C

PCR data Exp. ID 32643
Forward primer (5'-3') TGTGGGAGGCCCCAACCTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAGGTGCTCCATCTTCTAAAGC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1300	Approximate temperature 55°C

PCR data Exp. ID 33101
Forward primer (5'-3') TGTGGGAGGCCCCAACCTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAGGTGCTCCATCTTCTAAAGC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 1300	Approximate temperature 55°C

PCR data Exp. ID 33102
Forward primer (5'-3') TGTGGGAGGCCCCAACCTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAGGTGCTCCATCTTCTAAAGC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1300	Approximate temperature 55°C

PCR data Exp. ID 11437
Forward primer (5'-3') TGTGGGAGGCCCCAACCTGC	Digested band sizes (using EcoRV) Solanum lycopersicum LA925: 850+500 Solanum pennellii LA716: 1200	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAGGTGCTCCATCTTCTAAAGC	Enzymes All possible enzymes for pennellii and and lycopersicum: AluI, EcoRV
Accessions and product sizes Solanum lycopersicum LA925: 1350 Solanum pennellii LA716: 1350	Approximate temperature 55°C

Universal primers for Asterid species

No additional PCR data found.

Other COSII sequence data

Related Markers

Related Genotyped Markers: These markers have been genotyped and share a name with this marker

Genomic location of C2_At4g20410

%2Fsearch%2Fmarkers%2Fmarkerinfo.pl%3Fmarker_id%3D8307 marker 8307

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Accessions	Seedlots
Accessions		Breeding Program	Seedlot Name	Contents	Seedlot Location	Count	Weight(g)

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