Sol Genomics Network

SGN-M9058
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	308839 (SGN) 128801 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U197703	-	-	-
Lycopersicon Combined	Single	SGN-U240700	-	-	-
Solanum tuberosum	Single	SGN-U262643	-	-	-
Arabidopsis	Single	At5g53000 on TAIR	-	-	-

Map: Tobacco N. tomentosiformis
Map version 91 (location: 46777)
Chromosome 12
Position 79.90 cM
Confidence CF(LOD3)
Protocol CAPS

Tobacco N. tomentosiformis

Tomato-EXPEN 2000

PCR data Exp. ID 39992

Forward primer (5'-3')

TTGCTTGAAATGCTGAAGAAAGAGG

Accessions and product sizes

Solanum lycopersicum TA209

1000

Solanum pennellii TA56

1000

Approximate temperature

55°C

Reverse primer (5'-3')

AAGTAGCACAGGTGATAGGTGCTGC

Digested band sizes (using amplicon difference)

Solanum lycopersicum TA209

650

Solanum pennellii TA56

800

Mg⁺² concentration

1.5mM

Enzymes

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 12010
Forward primer (5'-3') TTGCTTGAAATGCTGAAGAAAGAGG		Mg⁺² concentration 3mM
Reverse primer (5'-3') AAGTAGCACAGGTGATAGGTGCTGC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 500	Approximate temperature 55°C

PCR data Exp. ID 12011
Forward primer (5'-3') TTGCTTGAAATGCTGAAGAAAGAGG		Mg⁺² concentration 3mM
Reverse primer (5'-3') AAGTAGCACAGGTGATAGGTGCTGC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 500	Approximate temperature 55°C

PCR data Exp. ID 12012
Forward primer (5'-3') TTGCTTGAAATGCTGAAGAAAGAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGTAGCACAGGTGATAGGTGCTGC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 500	Approximate temperature 55°C

PCR data Exp. ID 32585
Forward primer (5'-3') TTGCTTGAAATGCTGAAGAAAGAGG		Mg⁺² concentration 3mM
Reverse primer (5'-3') AAGTAGCACAGGTGATAGGTGCTGC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 500	Approximate temperature 55°C

PCR data Exp. ID 12013
Forward primer (5'-3') TTGCTTGAAATGCTGAAGAAAGAGG	Digested band sizes (using TaqI) Solanum pennellii LA716: 500 Solanum lycopersicum LA925: 400	Mg⁺² concentration 3mM
Reverse primer (5'-3') AAGTAGCACAGGTGATAGGTGCTGC	Enzymes All possible enzymes for pennellii and and lycopersicum: AluI,BsiHKAI,DpnII,TaqI
Accessions and product sizes Solanum pennellii LA716: 500 Solanum lycopersicum LA925: 500	Approximate temperature 55°C