Sol Genomics Network

SGN-M9074
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	303228 (SGN) 123802 (CGN)	-	-	-
Capsicum annuum	Multiple	SGN-U196747	-	-	-
Lycopersicon Combined	Single	SGN-U216168	-	-	-
Arabidopsis	Single	At5g54160 on TAIR	-	-	-

Arabidopsis COSII

PCR data Exp. ID 41865

Forward primer (5'-3')

TTCCGTTGCCCCACTTTTGC

Accessions and product sizes

Solanum lycopersicum TA209

1150

Solanum pennellii TA56

1100

Approximate temperature

55°C

Reverse primer (5'-3')

TTGAATGGGATTCCACCATCAAG

Digested band sizes (using amplicon difference)

Solanum pennellii TA56

1100

Solanum lycopersicum TA209

1150

Mg⁺² concentration

1.5mM

Enzymes

Other PCR data

PCR data Exp. ID 12017
Forward primer (5'-3') TTCCGTTGCCCCACTTTTGC		Mg⁺² concentration Unknown
Reverse primer (5'-3') TTGAATGGGATTCCACCATCAAG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 2500	Approximate temperature Unknown

PCR data Exp. ID 12331
Forward primer (5'-3') TGGAACTTATGGCTAAGGCTGGTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGCTTACAGTAGCCCCTGTTCCA	Enzymes
Accessions and product sizes Solanum melongena TA2986: Multiple	Approximate temperature 55°C

PCR data Exp. ID 12332
Forward primer (5'-3') TGGAACTTATGGCTAAGGCTGGTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGCTTACAGTAGCCCCTGTTCCA	Enzymes
Accessions and product sizes Capsicum annuum TA3428: 700	Approximate temperature 55°C

PCR data Exp. ID 12333
Forward primer (5'-3') TGGAACTTATGGCTAAGGCTGGTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGCTTACAGTAGCCCCTGTTCCA	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 700+1200	Approximate temperature 55°C

PCR data Exp. ID 12334
Forward primer (5'-3') TGGAACTTATGGCTAAGGCTGGTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGCTTACAGTAGCCCCTGTTCCA	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 700+1200	Approximate temperature 55°C

PCR data Exp. ID 12335
Forward primer (5'-3') TGGAACTTATGGCTAAGGCTGGTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGCTTACAGTAGCCCCTGTTCCA	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: Multiple	Approximate temperature 55°C

PCR data Exp. ID 12336
Forward primer (5'-3') TGGAACTTATGGCTAAGGCTGGTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGCTTACAGTAGCCCCTGTTCCA	Enzymes
Accessions and product sizes Physalis TA1367: 1200	Approximate temperature 55°C

PCR data Exp. ID 12337
Forward primer (5'-3') TGGAACTTATGGCTAAGGCTGGTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGCTTACAGTAGCCCCTGTTCCA	Enzymes
Accessions and product sizes Petunia axillaris subsp. parodii TA3310: 1300	Approximate temperature 55°C

PCR data Exp. ID 12338
Forward primer (5'-3') TGGAACTTATGGCTAAGGCTGGTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGCTTACAGTAGCCCCTGTTCCA	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: 1300	Approximate temperature 55°C

PCR data Exp. ID 12339
Forward primer (5'-3') TGGAACTTATGGCTAAGGCTGGTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGCTTACAGTAGCCCCTGTTCCA	Enzymes
Accessions and product sizes Coffea canephora BP409: 1000	Approximate temperature 55°C

PCR data Exp. ID 33364
Forward primer (5'-3') TGGAACTTATGGCTAAGGCTGGTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGCTTACAGTAGCCCCTGTTCCA	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 700+1200	Approximate temperature 55°C

PCR data Exp. ID 33365
Forward primer (5'-3') TGGAACTTATGGCTAAGGCTGGTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGCTTACAGTAGCCCCTGTTCCA	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 700+1200	Approximate temperature 55°C

PCR data Exp. ID 33366
Forward primer (5'-3') TGGAACTTATGGCTAAGGCTGGTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGCTTACAGTAGCCCCTGTTCCA	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: Multiple	Approximate temperature 55°C